Probing the Regiospecificity of Enzyme-Catalyzed Steroid Glycosylation
نویسندگان
چکیده
منابع مشابه
Multiple steroid-binding orientations: alteration of regiospecificity
The mutation of Ala-i 17 to Val conferred dehydroepiandrosterone (DHEA) hydroxylase activity on cytochrome P-450 2a-4, with the production of both 2aand 7ahydroxyDHEA at similar rates. P-450 2a-5 which has Val at position 117, acquired high DHEA hydroxylase activity by mutation of Phe-209. Mutant F209L of P-450 2a-5 exhibited strong regiospecificity at the 2-position of the DHEA molecule with t...
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ژورنال
عنوان ژورنال: Organic Letters
سال: 2012
ISSN: 1523-7060,1523-7052
DOI: 10.1021/ol3024924